Complete nucleotide sequence of an immunoglobulin heavy-chain gene and analysis of immunoglobulin gene organization in a primitive teleost species.
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چکیده
منابع مشابه
Complete nucleotide sequence of an immunoglobulin heavy-chain gene and analysis of immunoglobulin gene organization in a primitive teleost species.
The immunoglobulin heavy-chain variable region (VH) locus in a phylogenetically primitive teleost (Elops saurus) has been characterized by a strategy that relied initially on cross-hybridization between genomic VH segments and a murine VH probe. Using a homologous (Elops) VH probe and DNA sequencing, this gene family has been shown to be complex and to contain overt pseudogenes. A homologous pr...
متن کاملRegulation of immunoglobulin heavy-chain gene rearrangements.
Regulated assembly of antigen receptor gene segments to produce functional genes is a hallmark of B- and T-lymphocyte development. The immunoglobulin heavy-chain (IgH) and T-cell receptor beta-chain genes rearrange first in B and T lineages, respectively. Both loci require two recombination events to assemble functional genes; D-to-J recombination occurs first followed by V-to-DJ recombination....
متن کاملThe Complete Nucleotide Sequence of the Human Immunoglobulin Heavy Chain Variable Region Locus
The complete nucleotide sequence of the 957-kb DNA of the human immunoglobulin heavy chain variable (VH) region locus was determined and 43 novel VH segments were identified. The region contains 123 VH segments classifiable into seven different families, of which 79 are pseudogenes. Of the 44 VH segments with an open reading frame, 39 are expressed as heavy chain proteins and 1 as mRNA, while t...
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A germ-line heavy-chain variable region (VH) gene (RTVH431) has been isolated from a rainbow trout (Salmo gairdneri) and characterized by complete nucleotide sequencing. It is characteristic of VH, as shown by the conserved octamer and TATA motif in the 5' region, the heptamernonamer recombination signal sequence in the 3' region, and the 18-amino-acid-long hydrophobic leader interrupted by an ...
متن کاملThe complete nucleotide sequence of a human immunoglobulin genomic C mu gene.
We report here the DNA sequence of a genoraic Cv clone that includes the membrane exons. This clone among others was isolated by us from a HeLa cell library by screening with a DNA fragment containing a portion of exon 1 and exon 2 of the human Cfi gene which was derived from a cDNA clone of the human mu gene isolated in this laboratory. The sequence reported here is consistent with the partial...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1990
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.87.2.811